Zebrabiores group attended at the 10th Annual NGS & Clinical Diagnostics Congress in Warsaw, Poland. In this congress, PhD student Carlos Coppel, PhD student Alvaro Arana, PhD student Alba Pensado, PhD student Ana Quelle, PhD student Andrés Blanco presented a poster titled "Metaanalysis of different technical approaches in zebrafish models”.
Traditionally, mutant organisms have been widely used in various areas of research in order to test the function of genes involved in different biological processes. Random mutagenesis, based on mutagens such as ENU, has been the most used method to generate mutations in zebrafish. With the emergence of directed genome editing techniques: TALEN, Zinc Finger (ZFN) and especially CRISPR-Cas9, the generation of mutants has undergone an unprecedented revolution. On the other hand, reverse genetics techniques, being morpholinos the most used in zebrafish, have been questioned by the scientific community for its off-target effects and its low phenotypic correlation with mutants. Thus, in the present study a bibliographic review has been carried out, compiling a series of data from the different mutagenesis techniques. The aim is to cross these data and to learn about the efficacy of each technique and compare it with the efficacy derived from morpholinos.